Basic Sciences

Download An Atlas of Induced Sputum: An Aid for Research and by Ratko. Djukanovic, Peter Sterk PDF

By Ratko. Djukanovic, Peter Sterk

Containing greater than one hundred fifty photographs of the best quality, An Atlas of precipitated Sputum: An relief for study and prognosis is the 1st finished medical consultant at the program of this diagnostic strategy to non-infectious inflammatory ailments. The ebook contains contributions from prime specialists during this speedily starting to be box who have been individuals within the activity strength on brought about sputum backed by means of the eu breathing Society and chaired via the editors of this Atlas, Drs. Djukanovic and Sterk. providing a variety of graphs and illustrations, the atlas expertly describes suggestions and systems and gives transparent ideas for sputum induction and research according to the ecu respiration Society job strength document. A diagnostic reference for pulmonologists, the atlas does double accountability as an instructive textual content for citizens and physicians in education.

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Note the high background due to presence of mucus and the large number of squamous cells. Such inadequate sputum preparations make immunocytochemistry very difficult to interpret and should not be used CHAPTER 3 Analysis of soluble mediators in the sputum fluid phase Margaret Kelly and janis Shute Sputum contains viscous mucus that requires dispersion in order to release soluble mediators so that they can be measured. The large number of soluble mediators measured in sputum has been matched by the large variety of sputum processing techniques applied, even for the measurement of the same mediator; this and the use of different assay methods have made it difficult to compare measurements conducted in different centres.

For each measured mediator, the differences between the two measurements are plotted against the mean value of the two measurements. There was no difference in the levels of mediators in sputum induced by isotonic saline compared with induction by hypertonic saline. 7). Adapted with permission from Cataldo D, Foidart JM, Louis R, et al. Induced sputum: comparison between isotonic and hypertonic saline solution inhalation in patients with asthma. 8), but it must not be assumed that this applies to all other, as yet; unmeasured mediators whose release may be modulated by inhalation of hypertonic saline.

8 Repeatability of eosinophil cationic protein (ECP), albumin, and matrix metalloproteinase (MMP) measurements. This was assessed in 16 subjects who underwent sputum induction twice at weekly intervals with isotonic or hypertonic saline. A Bland-Altman graph is shown where, if the variable is reproducible, it is expected that 95% of the differences between measures are less than 2 standard deviations (SD). For each measured mediator, the differences between the two measurements are plotted against the mean value of the two measurements.

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